DNA

Part:BBa_K1791002:Design

Designed by: Graeme Glaister   Group: iGEM15_Lethbridge   (2015-09-17)


Low RBS expressing MS2 Coat protein ribozyme affinity purification (RAP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 605
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 97
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to consider MS2 expression levels relative to the theophylline ribozyme transcript and assessed how this affects total RNA yield. In order to test this we developed a second part with increased MS2 expression (BBa_K1791001)


Source

This is an amalgamation of several biobricks an MS2 coat protein, an MS2 binding domain and the theophylline ribozyme (BBa_K1791000)

References